The production of penicillin using fractions obtained from aqueous extracts of pea (Pisum sativum)

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The production of penicillin using fractions obtained from aqueous extracts of pea (Pisum sativum).

Aqueous extracts of air-dried ground peas% can provide a good mediuim for the growth and production of penicillin byPeniciUium notatum (Cook&Tulloch, 1944). The experiments to be described were carried out to determine what constituents of the pea were responsible for this effect. Although other vegetable extracts give a similar effect the pea lias been chosen as it is a readily available mater...

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Characterization of pea (Pisum sativum) seed protein fractions.

BACKGROUND Legume seed proteins have to be chemically characterized in order to properly link their nutritional effects with their chemical structure. RESULTS Vicilin and albumin fractions devoid of cross-contamination, as assessed by mass peptide fingerprinting analysis, were obtained from defatted pea (Pisum sativum cv. Bilbo) meal. The extracted protein fractions contained 56.7-67.7 g non-...

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Supaeromaculata Lines in Pea (Pisum sativum L.) Obtained Following Mutagenesis

Six pea (Pisum sativum L.) mutant lines characterized by more extensive expression of the patches of silver flecking (aeromaculata) were isolated following mutagenesis. Line III/122 demonstrated the strongest expression of the supaeromaculata phenotype; the silver flecks covered large area of leaf surface. The phenotype of 1/221, 2/123, 3/13, 2/1413 and X/3 was expressed clearly but quite weake...

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Role of nitrogen content of pea (Pisum sativum L.) on pea aphid (Acyrthosiphon pisum Harris) establishment

The leaf nitrogen content is generally accepted as an indicator of food quality and as a factor affecting host selection by phytophagous insects. The alate pea aphids (Acyrthosiphon pisum Harris, Aphididae) were given a choice among non-nodulated pea plants (Pisum sativum L.) supplied with one of four nitrate-N levels (0, 3, 15 and 30 mM). When whole plants were exposed to aphids for 7 days, th...

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Polyadenylate-Binding Protein from Pea (Pisum sativum)'

A polyadenylate-binding protein (PABP) was purified from cellfree extracts prepared from pea seedlings (Pisum sativum) by ammonium sulfate precipitation and Affi-Gel Blue and polyadenylate-Sepharose 4B affinity chromatography. The final preparation from polyadenylate-Sepharose 4B columns contained a single 70-kilodalton polypeptide with high polyadenylate-binding activity. The purified protein ...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1945

ISSN: 0306-3283

DOI: 10.1042/bj0390314